Lou Doillon started her acting career at the tender age of five as the daughter of her actress mother Jane Birkin’s character in the 1988 drama film ‘Kung-Fu Master!’ Ten years later, she had a starring role in her director father Jacques Doillon’s comedy film ‘Trop (peu) d'amour’ (Too Much (Little) Love). In 1999, she co-starred with Maud Forget as the lead characters of the Jean-Pierre Améris directed romantic drama ‘Mauvaises Fréquentations’ (Bad Company). The following year, she starred as Delphine in the film ‘Mamirolle’ and made her television debut in the episode “Avalanche” of the television series ‘Scénarios sur la drogue’ (Drug Scenes), a collection of 24 short films depicting drug abuse.
Lou Doillon again collaborated with her father in the 2001 comedy film ‘Carrément à l'ouest’ and played the eponymous character in the television series ‘Nadia Coupeau, dite Nana’ the same year. In 2002, she starred in Michel Blanc’s critically acclaimed French-Italian-British romantic comedy film ‘Embrassez qui vous voudrez’ (Summer Things) and played the titular role in the French adventure-comedy ‘Blanche’. In the following years, she had starring roles in the British telefilm ‘The Private Life of Samuel Pepys’ (2003), the French-Romanian horror film ‘Saint Ange’ (2004), and the Spanish comedy film ‘La vida perra de Juanita Narboni’ (The Wretched Life of Juanita Narboni) (2005). She subsequently acted in two independent English-language films: the horror thriller ‘Sisters’ (2006), which earned her a nomination at the Fangoria Chainsaw Awards as “Best Supporting Actress”, and the comedy-drama ‘Go Go Tales’ (2007). Also in 2007, she starred in her mother’s directorial debut ‘Les Boites’ (Boxes), which was based on the former’s own family life.
Lou Doillon was one of the main casts in the 2009 French drama ‘Bazar’, starring Bernadette Lafont. In 2010, she played the eponymous temptress in 1960s Paris in the Laure Charpentier directed French drama film ‘Gigola’ and starred in the short film ‘Le Crocodile Du Dniepr’, also known as ‘Dnipro Crocodile’. The same year, she made an appearance as herself in the episode “Belles de Jour” of the American teen drama series ‘Gossip Girl’, apart from appearances in the French television series ‘La collection pique sa crise’ and ‘Les invincibles’. In 2011, she had supporting roles in the French crime drama ‘Polisse’ and the British thriller ‘Moving Target’ (formerly known as Naked in London), acted in the short film ‘L'amère nature’, and appeared in all six episodes of the French adventure drama series ‘L'épervier’ (Sparrowhawk). She teamed up with her father again after a decade in the 2012 family drama ‘Un enfant de toi’ (A Child of Yours), following which she took a hiatus from acting to focus on her music career. Since then, she has appeared in Gia Coppola’s four-video series ‘The Myth Of Orpheus’ and ‘Eurydice’, a bold mixture of fashion film and narrative movie, produced for Vogue with Gucci.
Apart from her appearances in films and television series, Lou Doillon has acted alongside Michel Didym in the 2007 theatrical production of ‘Lettres Intimes’, a piece based on the correspondence of several famous people, at the Madeleine Theater in Paris for three months.