Holland Roden made her debut in the 2004 short film titled ‘Consideration.’ That same year, she also appeared in another short film ‘Back at the Ranch.’ She made her first television appearance in the Episode: "Goodbye and Good Luck" of the American procedural forensics crime drama television series ‘CSI: Crime Scene Investigation’ in 2007. The following year, she became a part of the television show ‘12 Miles of Bad Road.’ She appeared in 3 episodes of the show. In 2009, she starred as ‘Sky’ in the American teen comedy film ‘Bring It On: Fight to the Finish’ directed by Bille Woodruff. The film saw a direct-to-video release and received a mixed response from critics. She next appeared in television series like ‘Weeds’ (2009), ‘Community’ (2009), ‘Criminal Minds’ (2010), ‘The Event’ (2010), ‘Memphis Beat’ (2011). In 2011, she joined the main cast of the American supernatural teen drama television series ‘Teen Wolf’ where she played the role of ‘Lydia Martin.’ The show ran for 6 years and aired its final episode in 2017. Roden also bagged the ‘Teen Choice Award’ for her performance in the series.
In 2013, she starred as ‘Gabby’ in the supernatural thriller film ‘House of Dust’ directed by A.D. Calvo. The film received mixed to positive reviews from critics. She was next seen in television shows like ‘Fashion Police’ (2014) and ‘Lore’ (2017). In 2018, she starred as ‘Zoe Woods’ in the American horror anthology television series ‘Channel Zero’ created by Nick Antosca. Roden appeared in 6 episodes of the series. She played the role of ‘Erin Isaacs’ in the 2020 American adventure horror mystery film ‘No Escape’ directed by Will Wernick. The film saw negative reviews upon release. In 2021, she starred as ‘Rachel Ellis’ in the American psychological horror film ‘Escape Room: Tournament of Champions.’ The film received mixed to positive reviews from critics and was a box office success, grossing over $51.8 million on a budget of $15 million. That same year, she also appeared in the American crime film ‘Ted Bundy: American Boogeyman’ where she played the role of ‘Kathleen McChesney.’ In 2021, she also became a part of the American crime drama television series ‘Mayans M.C.’ The fourth season of the series will premiere in 2022. Roden is all set to appear in the upcoming film ‘The Re-Education of Molly Singer.’