Seo Ye-ji made her first screen appearance in the South Korean television sitcom ‘Potato Star 2013QR3’ in 2013. After making an appearance in ‘Diary of a Night Watchman’ (2014) and ‘Drama Special’ (2014), Ye-ji made her silver screen debut as ‘Queen Jeongsun’ in the 2015 South Korean historical drama film ‘The Throne’ directed by Lee Joon-ik. The film was selected to represent South Korea at the 88th Academy Awards for Best Foreign Language Film but could not make it to the final nomination. The Throne was also a commercial success and grossed over US$45.9 million on a budget of US$8.3 million. That same year, Ye-ji also starred in the South Korean mystery drama film ‘Circle of Atonement’ directed by Park Eun-gyoung and Lee Dong-ha. The film has its premiere at the Busan International Film Festival. She was next seen in films like ‘Seondal: The Man Who Sells the River’ (2016), ‘Another Way’ (2017), ‘The Bros’ (2017), ‘Stay With Me’ (2018), and ‘Warning: Do Not Play’ (2019). She portrayed ‘Seong Eun-yeong’ in the 2019 South Korean crime film ‘By Quantum Physics: A Nightlife Venture’ directed by Lee Seong-tae. Ye-ji won the ‘Buil Film Awards’ for her performance in the film.
In 2020, she grabbed the role of ‘Ko Mun-yeong’ in the South Korean television series ‘It's Okay to Not Be Okay’ created by Studio Dragon. The show received mostly positive reviews from critics. Ye-ji also won the ‘Asia Artist Award’ along with 2 ‘APAN Star Awards’ for her performance in the show. She was last seen playing the role of Lee La-el’ in the South Korean television series ‘Eve’ in 2022.